› 4dc1

X-ray diffraction
2.82Å resolution

Crystal Structure of Y202F Actinorhodin Polyketide Ketoreductase with NADPH

Released:
DOI: 10.2210/pdb4dc1/pdb
PDB entry 4dc1 coloured by chain, front view.
PDB entry 4dc1 coloured by chain, side view.
PDB entry 4dc1 coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
The determinants of activity and specificity in actinorhodin type II polyketide ketoreductase.
Javidpour P, Bruegger J, Srithahan S, Korman TP, Crump MP, Crosby J, Burkart MD, Tsai SC
Chem Biol 20 1225-34 (2013)
PMID: 24035284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ketoacyl reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 29.45 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16544 (Residues: 1-261; Coverage: 100%)
Gene names: SCBAC28G1.12c, SCO5086, actIII
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NDP 2 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 104.202Å b: 104.202Å c: 123.295Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.187 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Type II fatty acid and polyketide synthases: deciphering protein-protein and protein-substrate interactions.
Chen et al. (2018)
1 more

2 mentions without citation

Comprehensive evaluation of mapping complex traits in wheat using genome-wide association studies.
Saini et al. (2022)
1 more