PDBe 4dbl

X-ray diffraction
3.49Å resolution

Crystal structure of E159Q mutant of BtuCDF

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vitamin B12-binding protein Chains: E, J
Molecule details ›
Chains: E, J
Length: 255 amino acids
Theoretical weight: 28.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37028 (Residues: 22-266; Coverage: 100%)
Gene names: JW0154, b0158, btuF, yadT
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain
Vitamin B12 import ATP-binding protein BtuD Chains: C, D, H, I
Molecule details ›
Chains: C, D, H, I
Length: 249 amino acids
Theoretical weight: 27.1 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06611 (Residues: 1-249; Coverage: 100%)
Gene names: JW1699, b1709, btuD
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Vitamin B12 import system permease protein BtuC Chains: A, B, F, G
Molecule details ›
Chains: A, B, F, G
Length: 349 amino acids
Theoretical weight: 37.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06609 (Residues: 1-326; Coverage: 100%)
Gene names: JW1701, b1711, btuC
Sequence domains: FecCD transport family
Structure domains: ABC transporter involved in vitamin B12 uptake, BtuC

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 133.65Å b: 166.93Å c: 132.82Å
α: 90° β: 119.76° γ: 90°
R-values:
R R work R free
0.215 0.214 0.251
Expression system: Escherichia coli