› 4cvs

X-ray diffraction
1.39Å resolution

Structure of Apobacterioferritin Y45F variant

Released:
DOI: 10.2210/pdb4cvs/pdb
PDB entry 4cvs coloured by chain, front view.
PDB entry 4cvs coloured by chain, side view.
PDB entry 4cvs coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Photo-oxidation of tyrosine in a bio-engineered bacterioferritin 'reaction centre'-a protein model for artificial photosynthesis.
Hingorani K, Pace R, Whitney S, Murray JW, Smith P, Cheah MH, Wydrzynski T, Hillier W
Biochim Biophys Acta 1837 1821-34 (2014)
PMID: 25107631

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABD3 (Residues: 1-158; Coverage: 100%)
Gene names: JW3298, b3336, bfr
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand CD 3 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 103.49Å b: 27.84Å c: 56.76Å
α: 90° β: 119.18° γ: 90°
R-values:
R R work R free
0.204 0.203 0.225
Expression system: Escherichia coli

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Citations

1 review citation

The biological water-oxidizing complex at the nano-bio interface.
Najafpour et al. (2015)
5 other articles

3 mentions without citation

De novo protein design of photochemical reaction centers.
Ennist et al. (2022)
2 more