› 4cud

X-ray diffraction
1.85Å resolution

Human Notch1 EGF domains 11-13 mutant fucosylated at T466

Released:
DOI: 10.2210/pdb4cud/pdb
PDB entry 4cud coloured by chain, front view.
PDB entry 4cud coloured by chain, side view.
PDB entry 4cud coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fringe-mediated extension of O-linked fucose in the ligand-binding region of Notch1 increases binding to mammalian Notch ligands.
Taylor P, Takeuchi H, Sheppard D, Chillakuri C, Lea SM, Haltiwanger RS, Handford PA
Proc Natl Acad Sci U S A 111 7290-5 (2014)
PMID: 24803430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurogenic locus notch homolog protein 1 Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 14.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 410-526; Coverage: 5%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains: Laminin

Ligands and Environments

2 bound ligands:
Ligand FUC 1 x FUC
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3121
Unit cell:
a: 28.29Å b: 28.29Å c: 282.732Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.26
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The notch ligand JAGGED1 as a target for anti-tumor therapy.
Li et al. (2014)
14 more

4 mentions without citation

Fringe-mediated extension of O-linked fucose in the ligand-binding region of Notch1 increases binding to mammalian Notch ligands.
Taylor et al. (2014)
3 more