› 4cnz

X-ray diffraction
1.7Å resolution

Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine diphosphate

Released:
DOI: 10.2210/pdb4cnz/pdb
PDB entry 4cnz coloured by chain, front view.
PDB entry 4cnz coloured by chain, side view.
PDB entry 4cnz coloured by chain, top view.
Source organism: Azospirillum brasilense
Primary publication:
Structure and thermodynamics of effector molecule binding to the nitrogen signal transduction PII protein GlnZ from Azospirillum brasilense.
Truan D, Bjelić S, Li XD, Winkler FK
J Mol Biol 426 2783-99 (2014)
PMID: 24846646

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein P-II Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 112 amino acids
Theoretical weight: 12.29 KDa
Source organism: Azospirillum brasilense
UniProt:
  • Canonical: P70731 (Residues: 1-112; Coverage: 100%)
Gene names: C1S70_14085, CHT98_00165, D3093_10400, D3867_12440, D3868_07935, D3869_07130, FBZ82_105367, FBZ83_10540, FH063_005223, glnZ
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand ADP 6 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 64.324Å b: 87.35Å c: 119.693Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.231

Quick links

Citations

3 review citations

The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.
Huergo et al. (2015)
2 more

2 mentions without citation

Tuning the in vitro sensing and signaling properties of cyanobacterial PII protein by mutation of key residues.
Selim et al. (2019)
1 more