PDBe 4ch5

X-ray diffraction
2.2Å resolution

Structure of pyrrolysyl-tRNA synthetase in complex with adenylated propionyl lysine

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl- tRNA(Pyl). 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrrolysine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.42 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PWY1 (Residues: 184-454; Coverage: 60%)
Gene names: MM_1445, pylS
Sequence domains: tRNA synthetases class II core domain (F)
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P64
Unit cell:
a: 105.376Å b: 105.376Å c: 70.801Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.189
Expression system: Escherichia coli BL21(DE3)