› 4cgr

X-ray diffraction
2.1Å resolution

Structure of Regulator Protein SCO3201 from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb4cgr/pdb
PDB entry 4cgr coloured by chain, front view.
PDB entry 4cgr coloured by chain, side view.
PDB entry 4cgr coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structure and regulatory targets of SCO3201, a highly promiscuous TetR-like regulator of Streptomyces coelicolor M145.
Xu D, Waack P, Zhang Q, Werten S, Hinrichs W, Virolle MJ
Biochem Biophys Res Commun 450 513-8 (2014)
PMID: 24928397

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192175 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.45 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KYU4 (Residues: 23-236; Coverage: 91%)
Gene names: SCE22.18c, SCO3201
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 54.68Å b: 79.63Å c: 95.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.269
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Recent advances in understanding Streptomyces.
Chater KF. (2016)
2 other articles

1 mention without citation

Crystal structures of free and ligand-bound forms of the TetR/AcrR-like regulator SCO3201 from Streptomyces coelicolor suggest a novel allosteric mechanism.
Werten et al. (2023)