PDBe 4cej

X-ray diffraction
3Å resolution

Crystal structure of AddAB-DNA-ADPNP complex at 3 Angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
ATP-dependent helicase/nuclease subunit A Chain: A
Molecule details ›
Chain: A
Length: 1232 amino acids
Theoretical weight: 141.22 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli B
UniProt:
  • Canonical: P23478 (Residues: 1-1232; Coverage: 100%)
Gene names: BSU10630, addA
Sequence domains:
Structure domains:
ATP-dependent helicase/deoxyribonuclease subunit B Chain: B
Molecule details ›
Chain: B
Length: 1166 amino acids
Theoretical weight: 134.77 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli B
UniProt:
  • Canonical: P23477 (Residues: 1-1166; Coverage: 100%)
Gene names: BSU10620, addB
Sequence domains: PD-(D/E)XK nuclease superfamily
DNA Chain: X
Molecule details ›
Chain: X
Length: 70 nucleotides
Theoretical weight: 21.48 KDa

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 77.437Å b: 152.941Å c: 125.244Å
α: 90° β: 94.3° γ: 90°
R-values:
R R work R free
0.205 0.203 0.243
Expression system: Escherichia coli B