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Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Pol alpha.

X-ray diffraction
2.694Å resolution
PDB entry 4c93 coloured by chain, front view.
PDB entry 4c93 coloured by chain, side view.
PDB entry 4c93 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero pentamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA polymerase alpha catalytic subunit A and DNA polymerase alpha-binding protein
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Multimeric state: hetero pentamer
Accessible surface area: 49684.61 Å2
Buried surface area: 8703.33 Å2
Dissociation area: 1,139.6 Å2
Dissociation energy (ΔGdiss): 4.22 kcal/mol
Dissociation entropy (TΔSdiss): 14.48 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-146557

Macromolecules

DNA polymerase alpha-binding protein
Chains: A, B, C
Length: 478 amino acids
Theoretical weight: 54.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01454 (Residues: 471-927; Coverage: 49%)
Gene names: CHL15, CTF4, P9659.7, POB1, YPR135W
Pfam:
InterPro: Minichromosome loss protein Mcl1, middle region
DNA polymerase alpha-binding protein in PDB entry 4c93, assembly 1, front view.
DNA polymerase alpha-binding protein in PDB entry 4c93, assembly 1, side view.
DNA polymerase alpha-binding protein in PDB entry 4c93, assembly 1, top view.
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DNA polymerase alpha catalytic subunit A
Chains: D, E
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P13382 (Residues: 137-149; Coverage: 1%)
Gene names: CDC17, N2181, POL1, YNL102W
DNA polymerase alpha catalytic subunit A in PDB entry 4c93, assembly 1, front view.
DNA polymerase alpha catalytic subunit A in PDB entry 4c93, assembly 1, side view.
DNA polymerase alpha catalytic subunit A in PDB entry 4c93, assembly 1, top view.
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