PDBe 4c93

X-ray diffraction
2.69Å resolution

Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Pol alpha.

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase alpha-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 478 amino acids
Theoretical weight: 54.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01454 (Residues: 471-927; Coverage: 49%)
Gene names: CHL15, CTF4, P9659.7, POB1, YPR135W
Sequence domains: Minichromosome loss protein, Mcl1, middle region
DNA polymerase alpha catalytic subunit A Chains: D, E
Molecule details ›
Chains: D, E
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P13382 (Residues: 137-149; Coverage: 1%)
Gene names: CDC17, N2181, POL1, YNL102W

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 88.977Å b: 99.998Å c: 219.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided