› 4c6y

X-ray diffraction
1.8Å resolution

Ancestral PNCA (last common ancestors of Gram-positive and Gram- negative bacteria) beta-lactamase class A

Released:
DOI: 10.2210/pdb4c6y/pdb
PDB entry 4c6y coloured by chain, front view.
PDB entry 4c6y coloured by chain, side view.
PDB entry 4c6y coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins.
Risso VA, Gavira JA, Gaucher EA, Sanchez-Ruiz JM
Proteins 82 887-96 (2014)
PMID: 24710963

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BETA-LACTAMASE Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.09 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

6 bound ligands:
Ligand PG4 2 x PG4
Ligand PEG 21 x PEG
Ligand NA 2 x NA
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 39.581Å b: 57.473Å c: 240.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.142 0.17
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Protein stability: computation, sequence statistics, and new experimental methods.
Magliery TJ. (2015)
5 more

1 mention without citation

De novo active sites for resurrected Precambrian enzymes.
Risso et al. (2017)