4bx7

X-ray diffraction
2.26Å resolution

trans-divalent streptavidin bound to biotin-4-fluorescein

Released:
Source organism: Streptomyces avidinii
Primary publication:
Plug-and-play pairing via defined divalent streptavidins.
J Mol Biol 426 199-214 (2014)
PMID: 24056174

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149692 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.32 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like
Streptavidin Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 14.06 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3121
Unit cell:
a: 64.41Å b: 64.41Å c: 103.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.237
Expression system: Escherichia coli BL21(DE3)