› 4bwm

X-ray diffraction
1.75Å resolution

KlenTaq mutant in complex with a RNA/DNA hybrid

Released:
DOI: 10.2210/pdb4bwm/pdb
PDB entry 4bwm coloured by chain, front view.
PDB entry 4bwm coloured by chain, side view.
PDB entry 4bwm coloured by chain, top view.
Source organisms:
Primary publication:
Structure and function of an RNA-reading thermostable DNA polymerase.
Blatter N, Bergen K, Nolte O, Welte W, Diederichs K, Mayer J, Wieland M, Marx A
Angew Chem Int Ed Engl 52 11935-9 (2013)
PMID: 24106012

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117265 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
DNA polymerase I, thermostable Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 61.06 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19821 (Residues: 293-832; Coverage: 65%)
Gene names: pol1, polA
Sequence domains:
Structure domains:
5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*DOCP)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-R(*AP*AP*AP*GP*GP*GP*CP*GP*CP*CP*GP*UP*GP*GP*UP*C)-3' Chain: G
Molecule details ›
Chain: G
Length: 16 nucleotides
Theoretical weight: 5.19 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand DCT 1 x DCT
Ligand CL 2 x CL
Ligand MG 3 x MG
Ligand FMT 4 x FMT
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 143.192Å b: 86.282Å c: 63.094Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.208
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Modified nucleic acids: replication, evolution, and next-generation therapeutics.
Duffy et al. (2020)
2 more

4 mentions without citation

Engineering of a DNA Polymerase for Direct m6 A Sequencing.
Aschenbrenner et al. (2018)
3 more