PDBe 4buz

X-ray diffraction
1.9Å resolution

SIR2 COMPLEX STRUCTURE MIXTURE OF EX-527 INHIBITOR AND REACTION PRODUCTS OR OF REACTION SUBSTRATES P53 PEPTIDE AND NAD

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04637 (Residues: 379-386; Coverage: 2%)
Gene names: P53, TP53
NAD-dependent protein deacetylase Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.57 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WYW0 (Residues: 1-246; Coverage: 100%)
Gene names: TM_0490, cobB, sir2
Sequence domains: Sir2 family
Structure domains: SIR2/SIRT2 'Small Domain'

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 46.75Å b: 59.92Å c: 109.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.194
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)