PDBe 4bug

X-ray diffraction
2.8Å resolution

Pilus-presented adhesin, Spy0125 (Cpa), Cys426Ala mutant

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ancillary protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 51.37 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GRA2 (Residues: 285-723; Coverage: 58%)
Gene name: cpa1
Sequence domains: Thioester domain
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 45.96Å b: 116.86Å c: 176.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.268
Expression system: Escherichia coli BL21(DE3)