PDBe 4brk

X-ray diffraction
1.5Å resolution

Legionella pneumophila NTPDase1 N302Y variant crystal form III (closed) in complex with MG UMPPNP

Released:

Function and Biology Details

Reaction catalysed:
A nucleoside 5'-triphosphate + 2 H(2)O = a nucleoside 5'-phosphate + 2 phosphate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ectonucleoside triphosphate diphosphohydrolase I Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 41.93 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZUA2 (Residues: 35-393; Coverage: 91%)
Gene name: lpg1905
Sequence domains: GDA1/CD39 (nucleoside phosphatase) family
Structure domains: Exopolyphosphatase. Domain 2

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P212121
Unit cell:
a: 80.6Å b: 82.967Å c: 109.578Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.129 0.178
Expression system: Escherichia coli