EC 220.127.116.11: Apyrase
A nucleoside 5'-triphosphate + 2 H(2)O = a nucleoside 5'-phosphate + 2 phosphate.
- Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates.
- They differ from ATPases, which specifically hydrolyze ATP, by hydrolyzing both ATP and ADP.
- Most of the ecto-ATPases that occur on the cell surface and hydrolyze extracellular nucleotides belong to this enzyme family.
Nucleoside triphosphate phosphohydrolase (nucleoside monophosphoate-forming)
- ATP diphosphohydrolase
- Adenosine diphosphatase
Protein family (Pfam)
Domain description: GDA1/CD39 (nucleoside phosphatase) family
Domain description: Nucleoside phosphatase GDA1/CD39
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Nucleotidyltransferase; domain 5
Homology: Exopolyphosphatase. Domain 2Occurring in: