› 4br2

X-ray diffraction
2Å resolution

rat NTPDase2 in complex with Ca UMPPNP

Released:
DOI: 10.2210/pdb4br2/pdb
PDB entry 4br2 coloured by chain, front view.
PDB entry 4br2 coloured by chain, side view.
PDB entry 4br2 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystallographic snapshots along the reaction pathway of nucleoside triphosphate diphosphohydrolases.
Zebisch M, Krauss M, Schäfer P, Lauble P, Sträter N
Structure 21 1460-75 (2013)
PMID: 23830739

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ectonucleoside triphosphate diphosphohydrolase 2 Chain: A
Molecule details ›
Chain: A
Length: 457 amino acids
Theoretical weight: 50.7 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O35795 (Residues: 28-462; Coverage: 88%)
Gene names: Cd39l1, Entpd2
Sequence domains: GDA1/CD39 (nucleoside phosphatase) family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand UNP 1 x UNP
Ligand CA 1 x CA
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 41.58Å b: 69.02Å c: 164.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.201
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The use of polyoxometalates in protein crystallography - An attempt to widen a well-known bottleneck.
Bijelic et al. (2015)
4 more

2 mentions without citation

Isomeric chain structures of {[Mn(H₂O)₄]₂Ru₂(CO₃)₄Br₂}n(n-): syntheses, structural diversity and magnetic properties.
Hou et al. (2014)
1 more