Function and Biology

Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (FabG) from Pseudomonas aeruginosa in complex with 2-(trifluoromethyl)-1H- benzimidazole at 2.3A resolution

Source organism: Pseudomonas aeruginosa PAO1
Biochemical function: 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Biological process: oxidation-reduction process
Cellular component: not assigned

EC 1.1.1.100: 3-oxoacyl-[acyl-carrier-protein] reductase

Reaction catalysed:
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl- carrier-protein] + NADPH.
Comments:
  • Exhibits a marked preference for [acyl-carrier-protein] derivatives over CoA derivatives as substrates.
Systematic name:
(3R)-3-hydroxyacyl-[acyl-carrier-protein]:NADP(+) oxidoreductase
Alternative Name(s):
  • 3-ketoacyl acyl carrier protein reductase
  • 3-oxoacyl-[ACP]reductase
  • Beta-ketoacyl acyl carrier protein (ACP) reductase
  • Beta-ketoacyl reductase
  • Beta-ketoacyl thioester reductase
  • Beta-ketoacyl-ACP reductase
  • Beta-ketoacyl-[acyl-carrier protein](ACP) reductase
  • Beta-ketoacyl-acyl carrier protein reductase
  • NADPH-specific 3-oxoacyl-[acylcarrier protein]reductase

Sequence families

Pfam Protein families (Pfam)
PF00106
Domain description: short chain dehydrogenase
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG
1 copy of Pfam domain PF00106 (short chain dehydrogenase) in 3-oxoacyl-[acyl-carrier-protein] reductase FabG in PDB 4bnt.

PF08659
Domain description: KR domain
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG
1 copy of Pfam domain PF08659 (KR domain) in 3-oxoacyl-[acyl-carrier-protein] reductase FabG in PDB 4bnt.

PF13561
Domain description: Enoyl-(Acyl carrier protein) reductase
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG
1 copy of Pfam domain PF13561 (Enoyl-(Acyl carrier protein) reductase) in 3-oxoacyl-[acyl-carrier-protein] reductase FabG in PDB 4bnt.

InterPro InterPro annotations
IPR002347
Domain description: Short-chain dehydrogenase/reductase SDR
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG
IPR036291
Domain description: NAD(P)-binding domain superfamily
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG
IPR011284
Domain description: 3-oxoacyl-(acyl-carrier-protein) reductase
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG
IPR020904
Domain description: Short-chain dehydrogenase/reductase, conserved site
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG

Structure domain

CATH CATH domain
3.40.50.720
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: NAD(P)-binding Rossmann-like Domain
Occurring in:
  1. 3-oxoacyl-[acyl-carrier-protein] reductase FabG
1 copy of CATH domain 3.40.50.720 (Rossmann fold) in 3-oxoacyl-[acyl-carrier-protein] reductase FabG in PDB 4bnt.