PDBe 4bh6

X-ray diffraction
2.9Å resolution

Insights into degron recognition by APC coactivators from the structure of an Acm1-Cdh1 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
APC/C-CDH1 modulator 1 Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 70 amino acids
Theoretical weight: 8.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q08981 (Residues: 59-128; Coverage: 34%)
Gene names: ACM1, YPL267W
APC/C activator protein CDH1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 308 amino acids
Theoretical weight: 34.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53197 (Residues: 241-548; Coverage: 54%)
Gene names: CDH1, HCT1, YGL003C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 198.431Å b: 188.142Å c: 93.44Å
α: 90° β: 92.13° γ: 90°
R-values:
R R work R free
0.221 0.219 0.257
Expression system: Spodoptera frugiperda