PDBe 4bch

X-ray diffraction
2.96Å resolution

Structure of CDK9 in complex with cyclin T and a 2-amino-4-heteroaryl- pyrimidine inhibitor

Released:

Function and Biology Details

Reactions catalysed:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-T1 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 30.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60563 (Residues: 2-259; Coverage: 36%)
Gene name: CCNT1
Sequence domains: Cyclin, N-terminal domain
Structure domains: Cyclin-like
Cyclin-dependent kinase 9 Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 38.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50750 (Residues: 2-330; Coverage: 88%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R3
Unit cell:
a: 174.185Å b: 174.185Å c: 98.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.21
Expression system: Spodoptera frugiperda