› 4b43

X-ray diffraction
1.94Å resolution

Bacterial translation initiation factor IF2 (1-363), apo form, double mutant K86L H130A

Released:
DOI: 10.2210/pdb4b43/pdb
PDB entry 4b43 coloured by chain, front view.
PDB entry 4b43 coloured by chain, side view.
PDB entry 4b43 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Simonetti A, Urzhumtsev A, Klaholz BP

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation factor IF-2 Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.11 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48515 (Residues: 1-363; Coverage: 64%)
Gene names: TTHA0699, infB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GOL 4 x GOL
Ligand IOD 7 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 45.787Å b: 61.971Å c: 162.578Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.236
Expression system: Escherichia coli BL21(DE3)

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