PDBe 4b3k

X-ray diffraction
2.6Å resolution

Family 1 6-phospho-beta-D glycosidase from Streptococcus pyogenes


Function and Biology Details

Reactions catalysed:
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate. 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Putative beta-glucosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 479 amino acids
Theoretical weight: 55.04 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21
  • Canonical: Q99YP9 (Residues: 2-480; Coverage: 100%)
Gene name: SPy_1599
Structure domains: Glycosidases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 106.42Å b: 190.2Å c: 106.17Å
α: 90° β: 118.88° γ: 90°
R R work R free
0.226 0.223 0.286
Expression system: Escherichia coli BL21