› 4ak7

X-ray diffraction
1.8Å resolution

Crystal structure of BpGH117_E303Q in complex with neoagarobiose

Released:
DOI: 10.2210/pdb4ak7/pdb
PDB entry 4ak7 coloured by chain, front view.
PDB entry 4ak7 coloured by chain, side view.
PDB entry 4ak7 coloured by chain, top view.
Source organism: Phocaeicola plebeius
Primary publication:
Analysis of keystone enzyme in Agar hydrolysis provides insight into the degradation (of a polysaccharide from) red seaweeds.
Hehemann JH, Smyth L, Yadav A, Vocadlo DJ, Boraston AB
J Biol Chem 287 13985-95 (2012)
PMID: 22393053

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 45.95 KDa
Source organism: Phocaeicola plebeius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5CY74 (Residues: 22-402; Coverage: 99%)
Gene name: BACPLE_01671
Sequence domains: Glycosyl hydrolases family 43
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

6 bound ligands:
Ligand 47N 2 x 47N
Ligand CA 1 x CA
Ligand EDO 2 x EDO
Ligand ACT 2 x ACT
Ligand MG 2 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 83.9Å b: 93.55Å c: 104.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.155 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Microorganisms living on macroalgae: diversity, interactions, and biotechnological applications.
Martin et al. (2014)
6 more

3 mentions without citation

Dissecting conformational contributions to glycosidase catalysis and inhibition.
Speciale et al. (2014)
2 more