PDBe 4aez

X-ray diffraction
2.3Å resolution

Crystal Structure of Mitotic Checkpoint Complex

Released:
Source organism: Schizosaccharomyces pombe
Primary publication:
Structure of the mitotic checkpoint complex.
Nature 484 208-13 (2012)
PMID: 22437499

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
WD repeat-containing protein slp1 Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 401 amino acids
Theoretical weight: 43.98 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P78972 (Residues: 88-488; Coverage: 82%)
Gene names: SPAC821.08c, slp1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Mitotic spindle checkpoint component mad2 Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 203 amino acids
Theoretical weight: 23.48 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O14417 (Residues: 1-203; Coverage: 100%)
Gene names: SPBC20F10.06, mad2
Sequence domains: HORMA domain
Structure domains: HORMA domain
Mitotic spindle checkpoint component mad3 Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 223 amino acids
Theoretical weight: 26.38 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O59767 (Residues: 1-223; Coverage: 72%)
Gene names: SPCC1795.01c, SPCC895.02, mad3
Sequence domains: Mad3/BUB1 homology region 1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 70.81Å b: 286.9Å c: 72.01Å
α: 90° β: 119.04° γ: 90°
R-values:
R R work R free
0.222 0.221 0.267
Expression system: Trichoplusia ni