PDBe 4aay

X-ray diffraction
2.7Å resolution

Crystal Structure of the arsenite oxidase protein complex from Rhizobium species strain NT-26

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AroA Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 845 amino acids
Theoretical weight: 93.38 KDa
Source organism: arsenite-oxidising bacterium NT-26
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6VAL8 (Residues: 1-845; Coverage: 100%)
Gene name: aroA
Sequence domains:
Structure domains:
AroB Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 175 amino acids
Theoretical weight: 18.37 KDa
Source organism: arsenite-oxidising bacterium NT-26
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6VAL9 (Residues: 1-175; Coverage: 100%)
Gene name: aroB
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: 'Rieske'-like iron-sulphur domains

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 149.13Å b: 232.96Å c: 141.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.211
Expression system: Escherichia coli BL21(DE3)