PDBe 4a8l

X-ray diffraction
1.35Å resolution

Protein crystallization and microgravity: glucose isomerase crystals grown during the PCDF-PROTEIN mission

Released:
Source organism: Streptomyces rubiginosus
Entry authors: Decanniere K, Patino-Lopez L-D, Sleutel M, Evrard C, Van De Weerdt C, Haumont E, Gavira JA, Otalora F, Maes D

Function and Biology Details

Reaction catalysed:
D-xylopyranose = D-xylulose. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.28 KDa
Source organism: Streptomyces rubiginosus
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: I222
Unit cell:
a: 92.56Å b: 98.42Å c: 102.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.111 0.11 0.137