› 4a78

X-ray diffraction
2.01Å resolution

cytochrome c peroxidase M119W in complex with guiacol

Released:
DOI: 10.2210/pdb4a78/pdb
PDB entry 4a78 coloured by chain, front view.
PDB entry 4a78 coloured by chain, side view.
PDB entry 4a78 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of guaiacol and phenol bound to a heme peroxidase.
Murphy EJ, Metcalfe CL, Nnamchi C, Moody PC, Raven EL
FEBS J 279 1632-9 (2012)
PMID: 22093282

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c peroxidase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.79 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00431 (Residues: 68-361; Coverage: 81%)
Gene names: CCP, CCP1, CPO, YKR066C
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand JZ3 1 x JZ3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 51Å b: 75.35Å c: 106.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.232
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Challenging Density Functional Theory Calculations with Hemes and Porphyrins.
de Visser et al. (2016)
3 more