PDBe 4zt9

X-ray diffraction
3.1Å resolution

Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 1369 amino acids
Theoretical weight: 158.66 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
single-guide RNA Chains: B, D
Molecule details ›
Chains: B, D
Length: 85 nucleotides
Theoretical weight: 27.5 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 102.505Å b: 143.046Å c: 296.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.284
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli