PDBe 4znc

X-ray diffraction
2.28Å resolution

Fc fragment of human IgG in complex with the C domain of staphylococcal protein A mutant - Q9W

Released:
Primary publication:
Suppression of conformational heterogeneity at a protein-protein interface.
Proc. Natl. Acad. Sci. U.S.A. 112 9028-33 (2015)
PMID: 26157136

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin G-binding protein A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 58 amino acids
Theoretical weight: 6.7 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P38507 (Residues: 270-327; Coverage: 12%)
Gene name: spa
Sequence domains: B domain
Structure domains: Immunoglobulin FC, subunit C
Immunoglobulin heavy constant gamma 3 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 220 amino acids
Theoretical weight: 25.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P01860 (Residues: 168-377; Coverage: 56%)
Gene name: IGHG3
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 137.951Å b: 87.21Å c: 103.248Å
α: 90° β: 91.06° γ: 90°
R-values:
R R work R free
0.198 0.196 0.242
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli