PDBe 4zfg

X-ray diffraction
2.27Å resolution

Dual-specificity Fab 5A12 in complex with Angiopoietin 2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Angiopoietin-2 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O15123 (Residues: 277-496; Coverage: 46%)
Gene name: ANGPT2
Sequence domains: Fibrinogen beta and gamma chains, C-terminal globular domain
Structure domains:
Fragment antigen binding 5A12 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 24.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fragment antigen binding 5A12 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 181.668Å b: 109.292Å c: 43.946Å
α: 90° β: 100.47° γ: 90°
R-values:
R R work R free
0.179 0.177 0.223
Expression systems:
  • Trichoplusia ni
  • Escherichia coli