PDBe 4z2m

X-ray diffraction
2.98Å resolution

Crystal structure of human SPT16 Mid-AID/H3-H4 tetramer FACT Histone complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FACT complex subunit SPT16 Chain: B
Molecule details ›
Chain: B
Length: 287 amino acids
Theoretical weight: 33.37 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q9Y5B9 (Residues: 644-930; Coverage: 27%)
Gene names: FACT140, FACTP140, SUPT16H
Sequence domains: Histone chaperone Rttp106-like
Structure domains:
Histone H3.1 Chains: G, I
Molecule details ›
Chains: G, I
Length: 102 amino acids
Theoretical weight: 11.92 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P68431 (Residues: 35-136; Coverage: 75%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Structure domains: Histone, subunit A
Histone H4 Chains: H, J
Molecule details ›
Chains: H, J
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P62805 (Residues: 1-103; Coverage: 100%)
Gene names: H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Unit cell:
a: 65.442Å b: 69.784Å c: 187.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.19 0.246
Expression system: Baculovirus expression vector pFastBac1-HM