PDBe 4ysi

X-ray diffraction
1.02Å resolution

Structure of USP7 with a novel viral protein

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase 7 Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93009 (Residues: 63-205; Coverage: 13%)
Gene names: HAUSP, USP7
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
VIRF-1 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 687 Da
Source organism: Human gammaherpesvirus 8
Expression system: Not provided
UniProt:
  • Canonical: F5HF68 (Residues: 44-51; Coverage: 2%)
Gene name: vIRF-1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P41
Unit cell:
a: 69.975Å b: 69.975Å c: 45.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.154 0.16
Expression systems:
  • Escherichia coli
  • Not provided