PDBe 4yng

X-ray diffraction
2.28Å resolution

Twinned pyruvate kinase from E. coli in the T-state

Released:

Function and Biology Details

Reaction catalysed:
ATP + pyruvate = ADP + phosphoenolpyruvate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase I Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 470 amino acids
Theoretical weight: 50.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AD62 (Residues: 1-470; Coverage: 100%)
Gene names: ECs2383, Z2704, pykF
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 130.09Å b: 74.596Å c: 241.634Å
α: 90° β: 90.14° γ: 90°
R-values:
R R work R free
0.219 0.217 0.244
Expression system: Escherichia coli BL21(DE3)