PDBe 4ylq

X-ray diffraction
1.4Å resolution

Crystal Structure of a FVIIa-Trypsin Chimera (FT) in Complex with Soluble Tissue Factor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tissue factor Chain: T
Molecule details ›
Chain: T
Length: 219 amino acids
Theoretical weight: 24.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13726 (Residues: 33-251; Coverage: 83%)
Gene name: F3
Sequence domains: Interferon-alpha/beta receptor, fibronectin type III
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 152 amino acids
Theoretical weight: 17.49 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 61-212; Coverage: 34%)
Gene name: F7
Sequence domains:
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 249 amino acids
Theoretical weight: 27.51 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 56%)
Gene name: F7

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 71.31Å b: 80.04Å c: 123.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.174
Expression systems:
  • Escherichia coli BL21(DE3)
  • Cricetulus griseus