PDBe 4y6o

X-ray diffraction
1.6Å resolution

Human SIRT2 in complex with myristoylated peptide (TNF-alphaK20myr)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase sirtuin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 33.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IXJ6 (Residues: 52-356; Coverage: 75%)
  • Best match: Q8IXJ6-4 (Residues: 52-270)
Gene names: SIR2L, SIR2L2, SIRT2
Sequence domains: Sir2 family
Structure domains:
Intracellular domain 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 911 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01375 (Residues: 17-23; Coverage: 3%)
Gene names: TNF, TNFA, TNFSF2

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 37.136Å b: 48.785Å c: 96.96Å
α: 101.07° β: 91.33° γ: 112.27°
R-values:
R R work R free
0.208 0.206 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided