PDBe 4y5w

X-ray diffraction
3.1Å resolution

Transcription factor-DNA complex

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition by STAT6.
Proc. Natl. Acad. Sci. U.S.A. 113 13015-13020 (2016)
PMID: 27803324

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Signal transducer and activator of transcription 6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 549 amino acids
Theoretical weight: 61.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42226 (Residues: 113-658; Coverage: 65%)
  • Best match: P42226-2 (Residues: 3-484)
Gene name: STAT6
Sequence domains:
DNA (5'-D(P*AP*TP*GP*GP*AP*TP*TP*TP*CP*CP*TP*AP*GP*GP*AP*AP*GP*AP*CP*AP*A)-3') Chains: E, M
Molecule details ›
Chains: E, M
Length: 22 nucleotides
Theoretical weight: 6.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*TP*GP*TP*CP*TP*TP*CP*CP*TP*AP*GP*GP*AP*AP*AP*TP*CP*CP*AP*T)-3') Chains: F, N
Molecule details ›
Chains: F, N
Length: 22 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 68.298Å b: 94.241Å c: 147.785Å
α: 99.85° β: 101.73° γ: 89.93°
R-values:
R R work R free
0.251 0.25 0.278
Expression systems:
  • Escherichia coli
  • Not provided