PDBe 4y2g

X-ray diffraction
2.5Å resolution

Structure of BRCA1 BRCT domains in complex with Abraxas single phosphorylated peptide

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Breast cancer type 1 susceptibility protein Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 25.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38398 (Residues: 1646-1859; Coverage: 12%)
Gene names: BRCA1, RNF53
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
BRCA1-A complex subunit Abraxas 1 Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 938 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q6UWZ7 (Residues: 403-409; Coverage: 2%)
Gene names: ABRA1, ABRAXAS1, CCDC98, FAM175A, UNQ496/PRO1013

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 63.847Å b: 63.847Å c: 93.367Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.252
Expression systems:
  • Escherichia coli
  • Not provided