PDBe 4xr8

X-ray diffraction
2.25Å resolution

Crystal structure of the HPV16 E6/E6AP/p53 ternary complex at 2.25 A resolution


Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein; Ubiquitin-protein ligase E3A Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 41.8 KDa
Source organisms: Expression system: Escherichia coli K-12
  • Canonical: Q05086 (Residues: 406-417; Coverage: 1%)
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • nullnull
Gene names: E6AP, EPVE6AP, HPVE6A, JW3994, UBE3A, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Cellular tumor antigen p53 Chains: C, D
Molecule details ›
Chains: C, D
Length: 199 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
  • Canonical: P04637 (Residues: 94-292; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
Protein E6 Chains: F, H
Molecule details ›
Chains: F, H
Length: 151 amino acids
Theoretical weight: 18.3 KDa
Source organism: Human papillomavirus type 16
Expression system: Escherichia coli K-12
  • Canonical: P03126 (Residues: 8-158; Coverage: 96%)
Gene name: E6
Sequence domains: Early Protein (E6)
Structure domains: CRO Repressor

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 77.842Å b: 128.939Å c: 81.56Å
α: 90° β: 92.33° γ: 90°
R R work R free
0.196 0.194 0.246
Expression system: Escherichia coli K-12