PDBe 4xhu

X-ray diffraction
2.09Å resolution

The complex structure of Timeless_PAB and PARP-1_catalytic domain

Released:

Function and Biology Details

Reaction catalysed:
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Poly [ADP-ribose] polymerase 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 359 amino acids
Theoretical weight: 40.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09874 (Residues: 661-1014; Coverage: 35%)
Gene names: ADPRT, PARP1, PPOL
Sequence domains:
Structure domains:
Protein timeless homolog Chains: B, D
Molecule details ›
Chains: B, D
Length: 103 amino acids
Theoretical weight: 11.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNS1 (Residues: 1000-1098; Coverage: 8%)
Gene names: TIM, TIM1, TIMELESS, TIMELESS1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 99.364Å b: 98.416Å c: 116.9Å
α: 90° β: 113.19° γ: 90°
R-values:
R R work R free
0.183 0.182 0.21
Expression system: Escherichia coli