› 4x53

X-ray diffraction
2.3Å resolution

Structure of the class D Beta-Lactamase OXA-160 V130D in Acyl-Enzyme Complex with Aztreonam

Released:
DOI: 10.2210/pdb4x53/pdb
PDB entry 4x53 coloured by chain, front view.
PDB entry 4x53 coloured by chain, side view.
PDB entry 4x53 coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Structural basis of activity against aztreonam and extended spectrum cephalosporins for two carbapenem-hydrolyzing class D β-lactamases from Acinetobacter baumannii.
Mitchell JM, Clasman JR, June CM, Kaitany KC, LaFleur JR, Taracila MA, Klinger NV, Bonomo RA, Wymore T, Szarecka A, Powers RA, Leonard DA
Biochemistry 54 1976-87 (2015)
PMID: 25710192

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein transpeptidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.68 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D2XKK9 (Residues: 31-275; Coverage: 97%)
Gene name: blaOXA-160
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand AZR 1 x AZR
Ligand BCT 1 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 102.452Å b: 102.452Å c: 87.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

β-Lactam antibiotic targets and resistance mechanisms: from covalent inhibitors to substrates.
Mora-Ochomogo et al. (2021)
2 more

3 mentions without citation

Structural basis of activity against aztreonam and extended spectrum cephalosporins for two carbapenem-hydrolyzing class D β-lactamases from Acinetobacter baumannii.
Mitchell et al. (2015)
2 more