PDBe 4x23

X-ray diffraction
3.5Å resolution

CRYSTAL STRUCTURE OF CENP-C IN COMPLEX WITH THE NUCLEOSOME CORE PARTICLE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H4 Chains: B, F, L, P
Histone H2A Chains: C, G, M, Q
Molecule details ›
Chains: C, G, M, Q
Length: 102 amino acids
Theoretical weight: 11.09 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P84051 (Residues: 16-117; Coverage: 82%)
Gene names: CG31618, CG33808, CG33814, CG33817, CG33820, CG33823, CG33826, CG33829, CG33832, CG33835, CG33838, CG33841, CG33844, CG33847, CG33850, CG33862, CG33865, H2a, His2A, His2A:CG31618, His2A:CG33808, His2A:CG33814, His2A:CG33817, His2A:CG33820, His2A:CG33823, His2A:CG33826, His2A:CG33829, His2A:CG33832, His2A:CG33835, His2A:CG33838, His2A:CG33841, His2A:CG33844, His2A:CG33847, His2A:CG33850, His2A:CG33862, His2A:CG33865
Structure domains: Histone, subunit A
CENP-C Chains: U, V, W, X
Molecule details ›
Chains: U, V, W, X
Length: 25 amino acids
Theoretical weight: 3.22 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: Q66LH7 (Residues: 710-734; Coverage: 3%)
Gene names: Cenpc, Cenpc1
DNA (147-MER) Chains: I, S
Molecule details ›
Chains: I, S
Length: 147 nucleotides
Theoretical weight: 45.14 KDa
DNA (147-MER) Chains: J, T
Molecule details ›
Chains: J, T
Length: 147 nucleotides
Theoretical weight: 45.61 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D, NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 102.994Å b: 176.102Å c: 208.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.286
Expression systems:
  • Escherichia coli
  • Not provided