PDBe 4wyq

X-ray diffraction
3.2Å resolution

Crystal structure of the Dicer-TRBP interface

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Endoribonuclease Dicer Chains: A, D
Molecule details ›
Chains: A, D
Length: 123 amino acids
Theoretical weight: 14.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UPY3 (Residues: 267-389; Coverage: 6%)
Gene names: DICER, DICER1, HERNA, KIAA0928
RISC-loading complex subunit TARBP2 Chains: B, E
Molecule details ›
Chains: B, E
Length: 75 amino acids
Theoretical weight: 8.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15633 (Residues: 289-363; Coverage: 21%)
Gene names: TARBP2, TRBP
Structure domains: Double Stranded RNA Binding Domain
Poly(UNK) Chains: C, F
Molecule details ›
Chains: C, F
Length: 11 amino acids
Theoretical weight: 954 Da
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: F4132
Unit cell:
a: 291.91Å b: 291.91Å c: 291.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.259
Expression system: Escherichia coli BL21(DE3)