› 4wv6

X-ray diffraction
1.75Å resolution

Heterodimer of Importin alpha 1 with nuclear localization signal of TAF8

Released:
DOI: 10.2210/pdb4wv6/pdb
PDB entry 4wv6 coloured by chain, front view.
PDB entry 4wv6 coloured by chain, side view.
PDB entry 4wv6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules.
Trowitzsch S, Viola C, Scheer E, Conic S, Chavant V, Fournier M, Papai G, Ebong IO, Schaffitzel C, Zou J, Haffke M, Rappsilber J, Robinson CV, Schultz P, Tora L, Berger I
Nat Commun 6 6011 (2015)
PMID: 25586196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156496 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 50.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52292 (Residues: 60-529; Coverage: 89%)
Gene names: KPNA2, RCH1, SRP1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Transcription initiation factor TFIID subunit 8 Chains: B, C
Molecule details ›
Chains: B, C
Length: 14 amino acids
Theoretical weight: 1.67 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q7Z7C8 (Residues: 297-310; Coverage: 5%)
Gene names: TAF8, TAFII43, TBN

Ligands and Environments

1 bound ligand:
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 54.27Å b: 77.72Å c: 128.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.18
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
8 more

7 mentions without citation

Zooming in on Transcription Preinitiation.
Gupta et al. (2016)
6 more