PDBe 4wuu

X-ray diffraction
3.05Å resolution

Structure of ESK1 in complex with HLA-A*0201/WT1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
ESK1 Chain: D
Molecule details ›
Chain: D
Length: 216 amino acids
Theoretical weight: 22.75 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
IMMUNOGLOBULIN HEAVY CHAIN Chain: E
Molecule details ›
Chain: E
Length: 223 amino acids
Theoretical weight: 24.12 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Wilms tumor protein Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P19544 (Residues: 126-134; Coverage: 2%)
Gene name: WT1
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 34.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 70.08Å b: 118.26Å c: 126.302Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.198 0.254
Expression systems:
  • Cricetulus griseus
  • Escherichia coli
  • Not provided