› 4wtp

X-ray diffraction
1.3Å resolution

Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei

Released:
DOI: 10.2210/pdb4wtp/pdb
PDB entry 4wtp coloured by chain, front view.
PDB entry 4wtp coloured by chain, side view.
PDB entry 4wtp coloured by chain, top view.
Source organism: Rhizomucor miehei CAU432
Primary publication:
The first crystal structure of a glycoside hydrolase family 17 β-1,3-glucanosyltransferase displays a unique catalytic cleft.
Qin Z, Yan Q, Lei J, Yang S, Jiang Z, Wu S
Acta Crystallogr D Biol Crystallogr 71 1714-24 (2015)
PMID: 26249352

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exo-1,3-beta-glucanase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 32.74 KDa
Source organism: Rhizomucor miehei CAU432
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KKZ6 (Residues: 1-278; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 17

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 45.887Å b: 50.366Å c: 98.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Accurate Quantification of Laminarin in Marine Organic Matter with Enzymes from Marine Microbes.
Becker et al. (2017)
3 more

5 mentions without citation

Adaptive mechanisms that provide competitive advantages to marine bacteroidetes during microalgal blooms.
Unfried et al. (2018)
4 more