PDBe 4wni

X-ray diffraction
2.3Å resolution

Crystal structure of the T229K mutant of human GAPDH at 2.3 angstroems resolution


Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, O
Molecule details ›
Chains: A, B, C, O
Length: 335 amino acids
Theoretical weight: 36.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P04406 (Residues: 1-335; Coverage: 100%)
Gene names: CDABP0047, GAPD, GAPDH, OK/SW-cl.12
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NAD 3 x NAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 81.363Å b: 133.095Å c: 147.768Å
α: 90° β: 90° γ: 90°
R R work R free
0.169 0.169 0.198
Expression system: Escherichia coli BL21(DE3)