PDBe 4wl0

X-ray diffraction
2.89Å resolution

Ligand-free structure of human platelet phosphofructokinase in an R-state, crystal form I

Released:

Function and Biology Details

Reaction catalysed:
ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase, platelet type Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 761 amino acids
Theoretical weight: 83.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01813 (Residues: 26-762; Coverage: 94%)
Gene names: PFKF, PFKP
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 79.343Å b: 178.062Å c: 133.117Å
α: 90° β: 104.45° γ: 90°
R-values:
R R work R free
0.212 0.211 0.236
Expression system: Escherichia coli BL21(DE3)