PDBe 4wjg

X-ray diffraction
3.1Å resolution

Structure of T. brucei haptoglobin-hemoglobin receptor binding to human haptoglobin-hemoglobin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemoglobin subunit alpha Chains: A, F, K, P, U, Z
Molecule details ›
Chains: A, F, K, P, U, Z
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: 1, B, G, L, Q, V
Molecule details ›
Chains: 1, B, G, L, Q, V
Length: 146 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins
Haptoglobin Chains: 2, C, H, M, R, W
Molecule details ›
Chains: 2, C, H, M, R, W
Length: 315 amino acids
Theoretical weight: 35.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00738 (Residues: 92-406; Coverage: 81%)
Gene name: HP
Sequence domains: Trypsin
Structure domains:
Iron-regulated surface determinant protein H Chains: 3, D, I, N, S, X
Molecule details ›
Chains: 3, D, I, N, S, X
Length: 146 amino acids
Theoretical weight: 16.88 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99TD3 (Residues: 86-229; Coverage: 17%)
Gene names: SA1552, harA, isdH, sasI
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like
Haptoglobin-hemoglobin receptor Chains: 4, E, J, O, T, Y
Molecule details ›
Chains: 4, E, J, O, T, Y
Length: 343 amino acids
Theoretical weight: 36.85 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Komagataella pastoris
UniProt:
  • Canonical: I7BA80 (Residues: 36-378; Coverage: 93%)
Gene name: HpHbR
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 143.23Å b: 140.95Å c: 267.18Å
α: 90° β: 98.54° γ: 90°
R-values:
R R work R free
0.255 0.255 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Komagataella pastoris