PDBe 4w9n

X-ray diffraction
1.84Å resolution

Enoyl-acyl carrier protein-reductase domain from human fatty acid synthase complexed with triclosan

Released:

Function and Biology Details

Reactions catalysed:
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fatty acid synthase Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 339 amino acids
Theoretical weight: 36.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49327 (Residues: 1529-1867; Coverage: 14%)
Gene names: FAS, FASN
Sequence domains: Zinc-binding dehydrogenase
Structure domains:
Fatty acid synthase Chain: C
Molecule details ›
Chain: C
Length: 339 amino acids
Theoretical weight: 36.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49327 (Residues: 1529-1867; Coverage: 14%)
Gene names: FAS, FASN
Sequence domains: Zinc-binding dehydrogenase
Structure domains:

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P1
Unit cell:
a: 68.564Å b: 75.834Å c: 83.264Å
α: 65.38° β: 88.87° γ: 65.08°
R-values:
R R work R free
0.206 0.206 0.233
Expression system: Escherichia coli